- Arkham City Sirens: A loose collection of female heroes who safeguard Arkham City. The founding Sirens were Echo, Madcap and Mandragora.
- Chicago Police Department: The CPD has been a progressive unit, employing metahumans since early 2010. Their Redline Task Force has been key to stopping many crimes before they get out of hand.
- Esoteric Order: A group of occult-oriented individuals dedicating to guarding the island of R’lyeh, and preventing the rise of various Elder Gods.
- Federal Security Agency: A branch of the US Government , the FSA was specifically created to police the rise of supernatural and superhuman entities.
- GEM Force: Humanity doesn’t know much about the spacefaring organization that calls itself the GEM Force. What we do know is that they consider themselves a sort of intergalactic law enforcement agency who grant their operatives extraordinary powers using mystical gemstones.
- Lightning Corps: A department of the United Nations that deals with supernatural and superhuman threats. They are called upon as a last resort, and try to resolve matters nonlethally.
- Hellraisers: A group of occult-oriented villains devoted to corruption, general evildoing and chaos. They are typically responsible for the summoning of demons and the creation of hellmouths.
Neutral Organizations & Corporations
- Black League: A loosely affiliated group of occult-oriented individuals who came together to learn and share knowledge.
- Dragnov Industries: A megacorporation that manufactures everything from baby food to airplane parts.
- Orchid Corporation:
The Newhaven World didn’t progress in the same was as our Earth. Some nations fell, others rose from the ashes.
- Nubia: Located between Egypt and Sudan, Nubia is an ancient kingdom known for its rich deposits of gold, diamonds and other precious materials.
- Soviet Union: In this world, the Soviet Union never dissolved, and remains a world power to this day, thanks in part to the rise of Superwoman.
Arkham City, Massachusetts.
New York City, New York.
- Aliens: Though the public has no idea alien life exists, many in the government, and many in the vigilante business, know otherwise.
- Novas: Beings whose metagene has been activated by the stellar phenomena known as nova bombardment.
- Vampires: Undead beings who have to feed on blood to survive. Their curse gives them many weaknesses, but even more strengths. No two are the same.
- Wights: Humans who have undergone extensive psychic testing to give them powerful psionic abilities. Most of them work in secret for the Orchid Corporation.